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soluble α-cd28 37.51  (Thermo Fisher)


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    Thermo Fisher soluble α-cd28 37.51
    Soluble α Cd28 37.51, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/soluble+%CE%B1-cd28+37%2E51/anti+cd28/pmc04955995-93-12-16
    Average 90 stars, based on 1 article reviews
    soluble α-cd28 37.51 - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Diffuse traumatic brain injury induces prolonged immune dysregulation and potentiates hyperalgesia following a peripheral immune challenge
    Article Snippet: When indicated, 1 μM ATRA (Sigma-Aldrich, St. Louis, MO) or 0.5 mg/mL soluble α-CD28 (clone: 37.51, eBioscience, San Diego, CA) was added to the culture medium.



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    Treg cells stably express CD49b in a maturation-dependent manner. (A) Percentage of CD49b + cells among Treg cells stimulated in culture with <t>α-CD3/CD28</t> and IL-2 for 4 or 8 d. (B) Percentage of CD62L + or CD49b + cells among aTreg cells in tissues of 5-d-old or 8-wk-old mice. (C) Expression of Rag2 GFP in Treg subsets from 4-wk-old Rag2 GFP mice. (D) Quantification of C. **, P ≤ 0.01 by paired t test; ns, not significant. (E) The indicated Foxp3 GFP Treg subsets were separately transferred into sublethally irradiated congenic Foxp3 GFP mice. Shown is the expression of CD49b versus dilution of CTV by transferred Treg cells recovered from spleen after 6 d. (F) Quantification of CD49b expression by transferred Treg cells. Data are representative of two independent experiments. Bars depict means ± SD.
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    Treg cells stably express CD49b in a maturation-dependent manner. (A) Percentage of CD49b + cells among Treg cells stimulated in culture with <t>α-CD3/CD28</t> and IL-2 for 4 or 8 d. (B) Percentage of CD62L + or CD49b + cells among aTreg cells in tissues of 5-d-old or 8-wk-old mice. (C) Expression of Rag2 GFP in Treg subsets from 4-wk-old Rag2 GFP mice. (D) Quantification of C. **, P ≤ 0.01 by paired t test; ns, not significant. (E) The indicated Foxp3 GFP Treg subsets were separately transferred into sublethally irradiated congenic Foxp3 GFP mice. Shown is the expression of CD49b versus dilution of CTV by transferred Treg cells recovered from spleen after 6 d. (F) Quantification of CD49b expression by transferred Treg cells. Data are representative of two independent experiments. Bars depict means ± SD.
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    Average 90 stars, based on 1 article reviews
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    Treg cells stably express CD49b in a maturation-dependent manner. (A) Percentage of CD49b + cells among Treg cells stimulated in culture with <t>α-CD3/CD28</t> and IL-2 for 4 or 8 d. (B) Percentage of CD62L + or CD49b + cells among aTreg cells in tissues of 5-d-old or 8-wk-old mice. (C) Expression of Rag2 GFP in Treg subsets from 4-wk-old Rag2 GFP mice. (D) Quantification of C. **, P ≤ 0.01 by paired t test; ns, not significant. (E) The indicated Foxp3 GFP Treg subsets were separately transferred into sublethally irradiated congenic Foxp3 GFP mice. Shown is the expression of CD49b versus dilution of CTV by transferred Treg cells recovered from spleen after 6 d. (F) Quantification of CD49b expression by transferred Treg cells. Data are representative of two independent experiments. Bars depict means ± SD.
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    Treg cells stably express CD49b in a maturation-dependent manner. (A) Percentage of CD49b + cells among Treg cells stimulated in culture with <t>α-CD3/CD28</t> and IL-2 for 4 or 8 d. (B) Percentage of CD62L + or CD49b + cells among aTreg cells in tissues of 5-d-old or 8-wk-old mice. (C) Expression of Rag2 GFP in Treg subsets from 4-wk-old Rag2 GFP mice. (D) Quantification of C. **, P ≤ 0.01 by paired t test; ns, not significant. (E) The indicated Foxp3 GFP Treg subsets were separately transferred into sublethally irradiated congenic Foxp3 GFP mice. Shown is the expression of CD49b versus dilution of CTV by transferred Treg cells recovered from spleen after 6 d. (F) Quantification of CD49b expression by transferred Treg cells. Data are representative of two independent experiments. Bars depict means ± SD.
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    soluble α-cd28 mab 37.51 antibody  (Thermo Fisher)
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    Thermo Fisher soluble α-cd28 mab 37.51 antibody
    Treg cells stably express CD49b in a maturation-dependent manner. (A) Percentage of CD49b + cells among Treg cells stimulated in culture with <t>α-CD3/CD28</t> and IL-2 for 4 or 8 d. (B) Percentage of CD62L + or CD49b + cells among aTreg cells in tissues of 5-d-old or 8-wk-old mice. (C) Expression of Rag2 GFP in Treg subsets from 4-wk-old Rag2 GFP mice. (D) Quantification of C. **, P ≤ 0.01 by paired t test; ns, not significant. (E) The indicated Foxp3 GFP Treg subsets were separately transferred into sublethally irradiated congenic Foxp3 GFP mice. Shown is the expression of CD49b versus dilution of CTV by transferred Treg cells recovered from spleen after 6 d. (F) Quantification of CD49b expression by transferred Treg cells. Data are representative of two independent experiments. Bars depict means ± SD.
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    Treg cells stably express CD49b in a maturation-dependent manner. (A) Percentage of CD49b + cells among Treg cells stimulated in culture with α-CD3/CD28 and IL-2 for 4 or 8 d. (B) Percentage of CD62L + or CD49b + cells among aTreg cells in tissues of 5-d-old or 8-wk-old mice. (C) Expression of Rag2 GFP in Treg subsets from 4-wk-old Rag2 GFP mice. (D) Quantification of C. **, P ≤ 0.01 by paired t test; ns, not significant. (E) The indicated Foxp3 GFP Treg subsets were separately transferred into sublethally irradiated congenic Foxp3 GFP mice. Shown is the expression of CD49b versus dilution of CTV by transferred Treg cells recovered from spleen after 6 d. (F) Quantification of CD49b expression by transferred Treg cells. Data are representative of two independent experiments. Bars depict means ± SD.

    Journal: The Journal of Experimental Medicine

    Article Title: CD49b defines functionally mature Treg cells that survey skin and vascular tissues

    doi: 10.1084/jem.20181442

    Figure Lengend Snippet: Treg cells stably express CD49b in a maturation-dependent manner. (A) Percentage of CD49b + cells among Treg cells stimulated in culture with α-CD3/CD28 and IL-2 for 4 or 8 d. (B) Percentage of CD62L + or CD49b + cells among aTreg cells in tissues of 5-d-old or 8-wk-old mice. (C) Expression of Rag2 GFP in Treg subsets from 4-wk-old Rag2 GFP mice. (D) Quantification of C. **, P ≤ 0.01 by paired t test; ns, not significant. (E) The indicated Foxp3 GFP Treg subsets were separately transferred into sublethally irradiated congenic Foxp3 GFP mice. Shown is the expression of CD49b versus dilution of CTV by transferred Treg cells recovered from spleen after 6 d. (F) Quantification of CD49b expression by transferred Treg cells. Data are representative of two independent experiments. Bars depict means ± SD.

    Article Snippet: Single-cell suspensions were restimulated in U-bottom plates with 50 ng/ml PMA and 500 ng/ml ionomycin or 5 µg/ml soluble α-CD3 (clone 145-2C11; BioXCell) and 5 µg/ml soluble α-CD28 (clone 37.51; BioXCell), both in the presence of 1 µg/ml brefeldin A.

    Techniques: Stable Transfection, Expressing, Irradiation